Journal: Stem Cell Reports

Article Title: Histone methyltransferase Setd8 preserves chromatin accessibility to safeguard retinal progenitor cell identity during development

doi: 10.1016/j.stemcr.2025.102789

Figure Lengend Snippet: Setd8 deficiency reduces RPC proliferation and promotes apoptosis (A and D) Representative retinal section images of YFP (green) and EdU (magenta) staining at P0 (A) and P2 (D) from Ctrl (left) and Setd8 cKO (right) mice. Scale bars, 50 μm. (B and E) Quantification of the number of YFP+ (left graph) or EdU+ (right graph) cells at P0 (B) and P2 (E) ( n = 15 from 5 retinas). ∗∗ p < 0.01. (C and F) Proportion of YFP+ EdU+ (double-positive) cells among YFP+ cells at P0 (C) and P2 (F) ( n = 15 from 5 retinas). ∗∗ p < 0.01. (G) Representative retinal section images of γH2AX (magenta) staining at P0 (left) and P2 (right) from Ctrl (upper) and Setd8 cKO (lower) mice. Scale bars, 50 μm. (H) Quantification of the number of γH2AX + cells at P0 and P2 ( n = 15 from 5 retinas). (I) Representative retinal section images of YFP (green) and active caspase-3 (magenta) staining at P0 (left) and P2 (right) from Ctrl (upper) and Setd8 cKO (lower) mice. Scale bars, 50 μm. (J) Quantification of active caspase-3+ cells at P0 and P2 ( n = 15 from 5 retinas).

Article Snippet: The following primary antibodies were used: chicken anti-Nestin (1:500, NES, Aves Labs); goat anti-Sox2 (1:500, AF2018, R&D Systems); rabbit anti-active caspase 3 (1:500, AF-835, R&D Systems); chick anti-GFP (1:500, GFP-1010, Aves Labs); sheep anti-Chx10 (1:100, X1180P, Exalpha); rabbit anti-H4K20me1 (1:500, ab9051, Abcam); rabbit anti-S-opsin (1:500, AB5407, Millipore); rabbit anti-M-opsin (1:200, AB5405, Millipore); mouse anti-Brn3a (1:100, MAB1585, Millipore); mouse anti-Calbindin (1:500, CB300, Swant); goat anti-Otx2 (1:100, BAF1979, R&D Systems); mouse anti-Glutamine synthetase (1:500, 610517, BD Biosciences); rabbit anti-Pax6 (1:500, 901301, BioLegend); rabbit anti-γH2AX (1:500, ab2893, Abcam); and rat anti-BrdU (1:500, ab-6326, Abcam).

Techniques: Staining

Journal: The FASEB Journal

Article Title: Single‐cell sequencing reveals microglia induced angiogenesis by specific subsets of endothelial cells following spinal cord injury

doi: 10.1096/fj.202200337r

Figure Lengend Snippet: FIGURE 5 Immunohistochemical validation of the specific EC subtypes in the injury site. (A–C) Co-immunostaining of CD31 (green) and EC sub-cluster markers (red): Mt2A (EC3, A), Angpt2 (EC5, B), and Rgs5 (EC6, C), respectively, on spinal cord sections of SCI 3D, SCI 7D, and NC. Nuclei were stained with DAPI (blue). The area in the white box was selected to show in 3D view. Scale bar, 100 μm. (D–F) tSNE maps of the expression distribution of Mt2A (D), Angpt2 (E), and Rgs5 (F) in four samples.

Article Snippet: For immunostaining, the tissue sections were incubated with indicated primary antibodies overnight at 4°C: CD31 (R&D, AF3628, 1:200), GFAP (Abcam, ab4674, 1:500), IBA1 (Wako, 019– 19741, 1:500), αSMA (Cell Signaling Technologies, 19245S, 1:500), Mt2A (Thermo Fisher Scientific, MA1- 25479, 1:500), ANGPT2 (Proteintech, 24613– 1- AP, 1:100), RGS5 (Novus, NBP200880, 1:150).

Techniques: Immunohistochemical staining, Biomarker Discovery, Immunostaining, Staining, Expressing